By László Poppe, Csaba Paizs, Klaudia Kovács, Florin-Dan Irimie, Beáta Vértessy (auth.), Loredano Pollegioni, Stefano Servi (eds.)
Even although they're found in nature, non-proteinogenic amino acids are typically outlined as unnatural or non-natural. Beside their structural variety, curiosity in those compounds is because of their prevalence in nature, their organic houses, the analytical points, their use as probes, and their incorporation into peptides and proteins, between different purposes. Divided into 5 handy sections, Unnatural Amino Acids: equipment and Protocols offers with enzymatic tools used to provide non-natural amino acids, points in regards to the presence of unnatural amino acids in peptides with antimicrobial homes, genetic incorporation of unnatural amino acids into proteins (yeast and mammalian cells), and detection and quantification of D-amino acids and similar enzymes. Written within the hugely winning Methods in Molecular Biology™ sequence layout, chapters comprise introductions to their respective themes, lists of the required fabrics and reagents, step by step, with no trouble reproducible laboratory protocols, and notes on troubleshooting and heading off recognized pitfalls.
Authoritative and obtainable, Unnatural Amino Acids: equipment and Protocols serves as a great advisor for scientists and contributes to directing the eye of researchers to the numerous fields of starting to be medical curiosity in non-natural amino acids.
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Extra resources for Unnatural Amino Acids: Methods and Protocols
Deracemization by Stereo-Inversion 1. All reagents and solvents are purchased from Sigma-Aldrich. 2. 1H NMR Spectra are recorded on Bruker ARX 400 instrument operating at the 1H resonance frequency of 400 MHz. Chemical shifts (d, ppm) are reported relative to tetramethylsilane (TMS) as an internal standard. All spectra are recorded in DMSO-d6 (when not differently indicated) at 305 K. 3. Silica gel 60 F254 plates (Merck) are used for analytical TLC. Detection is achieved with UV light followed by I2, ninhydrin or potassium permanganate staining.
Wu B, Szymaāski W, Wietzes P et al. (2009) Enzymatic synthesis of enantiopure D- and E-amino acids by phenylalanine aminomutasecatalysed amination of cinnamic acid derivatives. ChemBioChem 10, 338–344. 52. Wu B, Szymaāski W, Wildeman S et al. (2010) Efﬁcient tandem biocatalytic process for the kinetic resolution of aromatic E-amino Acids. Adv Synth Catal 352, 1409–1412. 53. Eckhart L, Schmidt M, Mildner M et al. (2008) Histidase expression in human epidermal keratinocytes: Regulation by differentiation status and all-trans retinoic acid.
J Am Chem Soc 129, 6988–6989. 51. Wu B, Szymaāski W, Wietzes P et al. (2009) Enzymatic synthesis of enantiopure D- and E-amino acids by phenylalanine aminomutasecatalysed amination of cinnamic acid derivatives. ChemBioChem 10, 338–344. 52. Wu B, Szymaāski W, Wildeman S et al. (2010) Efﬁcient tandem biocatalytic process for the kinetic resolution of aromatic E-amino Acids. Adv Synth Catal 352, 1409–1412. 53. Eckhart L, Schmidt M, Mildner M et al. (2008) Histidase expression in human epidermal keratinocytes: Regulation by differentiation status and all-trans retinoic acid.