Epithelial Transport: A guide to methods and experimental by Simon A. Lewis (auth.), Nancy K. Wills PhD, Luis Reuss MD,

By Simon A. Lewis (auth.), Nancy K. Wills PhD, Luis Reuss MD, Simon A. Lewis PhD (eds.)

Building from common rules, the authors in actual fact clarify the elemental position of epithelia in plasma electrolyte and water stability. Emphasis is put on experimental techniques and method. A finished thesaurus of phrases is included.

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The Na+ is recycled across the basolateral membrane. Electroneutrality for transepithelial transport is maintained by Na+ secretion through the tight junctions. In both instances, the NKCC performs secondary-active Cl- transport, which raises intracellular [Cl-] to levels above those predicted for electrochemical equilibrium. Thus, Cl- can then exit the cell by electrodiffusion via channels across the opposite membrane. Regulation of NKCC (Haas, 1994) appears to involve changes in the density of active transporters in the membrane.

W. Seldin and G. Giebisch), Raven Press, New York, pp. 317-60. 2 Epithelial transport proteins Luis Reuss, Nancy K. Wills and Simon A. 6 Water channels 44 References 46 Epithelia separate fluid compartments within the body and transport ions, inorganic solutes and water between these compartments. Active transport occurs through the cells and is primarily dependent upon the operation of so-called ion pumps (primary active transport). These pumps create chemical or electrochemical driving forces that cause the passive movement of a single substance (passive transport via carriers or channels) or the coupled movement of two or more substances (secondary active transport via carriers).

2 Epithelial transport proteins Luis Reuss, Nancy K. Wills and Simon A. 6 Water channels 44 References 46 Epithelia separate fluid compartments within the body and transport ions, inorganic solutes and water between these compartments. Active transport occurs through the cells and is primarily dependent upon the operation of so-called ion pumps (primary active transport). These pumps create chemical or electrochemical driving forces that cause the passive movement of a single substance (passive transport via carriers or channels) or the coupled movement of two or more substances (secondary active transport via carriers).

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