DNA Profiling of Horse Urine Samples to Confirm Donor by Paula Hawthorne, Jenny Wang-Holmes, Judy Cawdell-Smith, Ann

By Paula Hawthorne, Jenny Wang-Holmes, Judy Cawdell-Smith, Ann E.O. Trezise

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Thus 1–3% of the genome of the rat (Rattus norvegicus) is centromeric satellite DNA, whereas it is 8% in the mouse (Mus musculus) and greater than 23% in the cow (Bovis domesticus). The length of the satellite repeat unit varies from the d(AT)n : d(TA)n structure found in the land crab (Gecarcinus lateratis) to the very complicated structure seen in the domestic cow (Fig. 15). Even within a single genus each species can have a distinctive set of satellite sequences. In general, little is known about the detailed structure of repeated DNA arrays but one family of repeated DNA that has Hf Hf 359 bp Hf Hf Hin fI digest Electrophoresis 359 bp Hf Hf 359 bp Ha Hf Ha Hf Hf Ha HinfI Hf Ha Hae III Electrophoresis 359 bp 1077 bp 718 bp 359 bp Fig.

2). The duration of the applied electrical pulses is varied from 1 to 90 s permitting separation of DNAs with sizes from 30 kb to 2 Mb. The mechanism by which this occurs is believed to be as follows. When a large DNA molecule enters a gel in response to an electrical field it must elongate parallel to the field. This field then is cut off and a new field applied at right angles to the long axis of the DNA. The molecule is unable to move until it reorientates in a new direction and the time required for this will depend on molecular weight.

Later Bellanné-Chantelot et al. (1992) constructed a human library with an average insert size of 810 kb and with some inserts as large as 1800 kb. There are a number of operational problems associated with the use of YACs (Kouprina et al. 1994; Monaco & Larin 1994). e. sequences from different regions of the genome cloned into a single YAC. Chimaeras may arise by co-ligation of DNA inserts in vitro prior to yeast transformation, or by recombination between two DNA molecules that were introduced into the same yeast cell.

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