Biochemisrty & Genetics by Golder Wilson

By Golder Wilson

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The answer is d. (Murray, pp 412–434. Scriver, pp 3–45. Sack, pp 3–29. ) Like bacterial DNA, eukaryotic DNA is replicated in a semiconservative manner. However, in contrast to most bacterial DNA, which is circular in structure, nuclear chromosomal DNA is a single, uninterrupted molecule that is linear and unbranched. A eukaryotic chromosome contains a strand of DNA at least 100 times as large as the DNA molecules found in prokaryotes. Eukaryotic, but not prokaryotic, DNA molecules are bound to small basic proteins called histones.

A poly(A) tail is added to the primary transcript after transcription, which facilitates transport and processing from the nucleus. The discovery of introns complicated Mendel’s idea of the gene as the smallest hereditary unit; a modern definition might be the colinear sequence of exons, introns, and adjacent regulatory sequences that accomplish protein expression. Using these principles, one can determine the size of the stimulin gene. It contains a coding region of 300 bp (100 amino acids × 3 bp per amino acid), plus 100 bp in the intron, plus 70 + 30 = 100 bp in the untranslated regions (total = 500 bp).

E. Conjugation Colinearity Recombination Transformation Transduction 18. Following ultraviolet damage of DNA in skin a. b. c. d. e. qxd 16 11/14/01 10:36 AM Page 16 Biochemistry and Genetics 19. Which of the following statements correctly describes eukaryotic nuclear chromosomal DNA? a. Each discontinuous piece making up the chromosomes of eukaryotes is about the same size as each prokaryotic chromosome b. Unlike bacterial DNA, no histones are associated with it c. It is not replicated semiconservatively d.

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